虞沂

1996.9 u2013 2002.6 华中农业大学生物技术基地班，本硕连读

2003.9 u2013 2007.3 中科院微生物研究所，博士

2007.7 - 2009. 6 上海交通大学生命科学技术学院生物工程系，讲师

2009.7 - 2010. 6华中农业大学生命科学技术学院，讲师

2010.7 u2013 2016.11武汉大学药学院，副教授

2012.8 u2013 2013.8美国Washington University in Saint Louis,访问学者

2016.11武汉大学药学院，教授

微生物来源天然产物的组合生物合成。

研究兴趣与方向:以天然产物为基础研制和开发新药一直是国际化学界和医药界共同关注的重点领域。天然产物的生物合成就是由小分子的简单化合物作为底物，经过一系列顺序催化反应，最终形成复杂化学结构的过程。本人的研究兴趣在于通过代谢工程和组合生物合成研究复杂天然产物的生物合成，即利用基因工程技术，有目的地对细胞代谢途径进行精确的修饰或改造，以提高目的天然产物活性或产量、或合成新的代谢产物；或是通过操纵天然产物生物合成途径中的基因来产生非天然的天然产物类似物库，并从中发现和发展更具应用价值的药物。

承担课题与经费：

2010年度武汉市青年科技晨光计划，6万
2010年度教育部新教师基金，3万

2011年国家自然科学基金，25万

2011年973子课题，26万

2014年973子课题，28.8万
2015年国家自然科学基金，78万

2017年武汉大学自主科研-拔尖创新人才，15万

科研成果:

1. Su Li, Zhang R, Kyeremeh K, Deng Z, Deng H* andYu Y*. Dissection of the neocarazostatin A C4 alkyl side chain biosynthesis by in vitro reconstitution.Organic & Biomolecular Chemistry, 2017, 15(18): 3843-3848

2. Su Li, Lv Meinan, Kyeremeh Kwaku, Deng Zixin, Deng Hai andYu Yi *.Organic & Biomolecular Chemistry, 2016 (14): 8679-8684

3. Lv M, Ji X, Zhao J, Li Y, Zhang C, Su L, Ding W, Deng Z,Yu Yi*and Zhang Q*. Characterization of the biosynthetic gene cluster for benzoxazole antibiotics A33853 reveals unusual assembly logic.J AM CHEM SOC,2016, 138(20):6427-35

4. Huang S., Elsayed S., Lv M., Tabudravu J., Rateb M., Gyampoh R., Kyeremyh K., Ebel R., Jaspars M., Deng Z.,Yu Yi* and Deng H.* Biosynthesis of neocarazostatin A reveals the sequential carbazole prenylation and hydroxylation in the tailoring steps.Chemistry & Biology, 2015, 22(12):1633-42

5. Lv Meinan, Zhao Junfeng, Deng Zixin andYu Yi*. Characterization of the biosynthetic gene cluster for benzoxazole antibiotics A33853 reveals unusual assembly logic.Chemistry & Biology, 2015, 22(10):1313-1324.

6. Huang S, Tabudravu J, Elsayed SS, Travert J, Peace D, Tong MH, Kyeremeh K, Kelly SM, Trembleau L, Ebel R, Jaspars M,Yu Yi*and Deng H*. Discovery of a Single Monooxygenase that Catalyzes Carbamate Formation and Ring Contraction in the Biosynthesis of the Legonmycins.Angew Chem Int Ed Engl.2015, 54(43):12697-12701

7. Huang S, Tong MH, Qin Z, Deng Z, Deng H,Yu Y.*. Identification and characterization of the biosynthetic gene cluster of thiolutin, a tumor angiogenesis inhibitor, inSaccharothrix algeriensisNRRL B-24137.Anticancer Agents Med Chem, 2015, 15(3):277-84.

8. Long Ma, Axel Bartholome, Ming Him Tong, Zhiwei Qin,Yi Yu, Thomas Shepherd, Kwaku Kyeremeh, Hai Deng* and David OHagan*. Identification of a fluorometabolite fromStreptomycessp. MA37: (2R3S4S)-5-fluoro-2,3,4-tri

hydroxypentanoic acid.Chemical Science, 2015, 6(2):1414-1419

9. Hao C.L., Huang S., Deng Z.X., Zhao C.M.,Yu Y.*Mining of the pyrrolamide antibiotics analogs in Streptomyces netropsis reveals the amidohydrolase-dependent "iterative strategy" underlying the pyrrole polymerization.PLOS ONE,2014, 9(6):e99077.

10. Qin Zhiwei, Wang Xiaoling, Rateb Mostafa, Assad Lina, Jaspars Marcel, Deng Zixin,Yu Yi, Deng Hai*. Disruption of a Methyltransferase Gene in Actinomycin G Gene Cluster in Streptomyces iakyrus Increases the Production of Phenazinomycin.FEMS Microbiology Letters,2014, 352(1): 62-8

11. Sheng Huang, Long Ma, Ming Him Tong,Yi Yu*, David OHagan and Hai Deng* Fluoroacetate biosynthesis from the marine-derived bacteriumStreptomyces xinghaiensisNRRL B-24674.Org Biomol Chem, 2014, 12(27): 4828-4831

12. Varman, A.M.*,Yu, Y.*, You L., Tang, Y.J. Photoautotrophic production of D-lactate in an engineered cyanobacterium.Microbial Cell Factories,2013, 12: 117 (*Contributed equally)

13. Qin, Z., Huang, S.,Yu, Y.*, Deng, H*.Dithiolopyrrolone natural products: isolation, synthesis and biosynthesis.Marine Drugs,2013, 11(10):3970-97

14.Yu, Y., You, L., Liu, D.Y., Hollinshead, W., Tang, Y.J., Zhang, F.Z. Development ofSynechocystissp. PCC 6803 as a Phototrophic Cell Factory.Marine Drugs,2013, 11(8):2894-916

15. Wang, X., Tabudravu, J., Rateb, M.E., Annand, K.J., Qin, Z., Jaspars, M., Deng, Z.,Yu, Y.*, Deng, H*. Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus.Molecular Biosystems,2013, 9(6):1286-9

16. Wu XM,Yu Y., Han LB, Li CL, Wang HY, Zhong NQ, Yao Y, Xia GX*.The tobacco BLADE-ON-PETIOLE2 gene mediates differentiation of the corolla abscission zone by controlling longitudinal cell expansion.Plant Physiology,2012, 159(2):835-50

17. Zhang, Q.;Yu, Y.*, Thioether Crosslinkages Created by a Radical SAM Enzyme.ChemBioChem,2012, 13(8), 1097-1099

18. Huang, S.; Zhao, Y.D.; Qin, Z.W.; Wang, X.L.; Onega, M.; Chen, L.; He, J.;Yu, Y.*; Deng, H.*, Identification and heterologous expression of the biosynthetic gene cluster for holomycin produced by Streptomyces clavuligerus.Process Biochemistry,2011, 46, 811-16（*Co-Corresponding Author）

19. Zhang, Q.; Li, Y.X.; Chen, D.D.;Yu, Y.; Duan, L.; Shen, B.; Liu, W. Radical-mediated enzymatic carbon chain fragmentation-recombination.Nature Chemical Biology,2011, 7, 154-60

20.Yu, Y.; Guo, H.; Zhang, Q.; Duan, L.; Ding, Y.; Liao, R.J.; Lei, C.; Shen, B.; Liu, W. NosA Catalyzing Carboxyl-Terminal Amide Formation in Nosiheptide Maturation via an Enamine Dealkylation on the Serine-Extended Precursor Peptide.J AM CHEM SOC,2010, 132(46), 16324-26.

21. Wang, J.;Yu, Y.; Tang, K.; Liu, W.; He, X.; Huang, X.; Deng, Z. Thiopeptide antibiotic cyclothiazomycin: Identification and Analysis of its biosynthetic gene cluster in Streptomyces hygroscopicus 10-22.(2010)Appl Environ Microb, 76(7), 2335-44.

22.Ding, Y.;Yu, Y.; Pan, H.; Guo, H.; Li, Y.; Liu, W.Moving posttranslational modifications forward to biosynthesize the glycosylated thiopeptide nocathiacin I in Nocardia sp. ATCC202099. (2010)Molecular Biosystems, 6(7), 1180-85.

23.Yu, Y.; Duan, L.; Zhang, Q.; Liao, R. J.; Ding, Y.; Pan, H. X.; Wendt-Pienkowski, E.; Tang, G. L.; Shen, B.; Liu, W., Nosiheptide Biosynthesis Featuring a Unique Indole Side Ring Formation on the Characteristic Thiopeptide Framework. (2009)ACS Chem Biol, 4(10), 855-64.

24. Liao, R. J.; Duan, L.; Lei, C.; Pan, H. X.; Ding, Y.; Zhang, Q.; Chen, D. J.; Shen, B.;Yu, Y.*; Liu, W.* Thiopeptide Biosynthesis Featuring Ribosomally SynthesizedPrecursor Peptides and Conserved Posttranslational Modifications. (2009)Chem Biol, 16 (2), 141-47.（*Co-Corresponding Author）

25. Liu, W.;Yu, Y.Combinatorial Biosynthesis of Pharmaceutical Natural Products. Biocatalysis for the Pharmaceutical Industry: Discovery, Development, and Manufacturing (Chapter 11)Wiley Press, ISBN: 978-0-470-82314-9, Oct. 2008, 229-246

26.Yu, Y.; Wang, H.Y.; Liu, L.N.; Chen, Z.L.; Xia, G.X. Functional identification of cytokinesis-related genes from tobacco BY-2 cells. (2007)Plant Cell Rep, 26(7), 889-94

27. Chen, Z.L.#;Yu, Y.#; Liu, L.N.; Xia, G.X. Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells. (2007)Progress in Nature Science17, 156-62. (*Co-First Author)

28.Yu, Y.; Bai, L. Q.; Minagawa, K.; Jian, X. H.; Li, L.; Li, J. L.; Chen, S. Y.; Cao, E. H.; Mahmud, T.; Floss, H. G.; Zhou, X. F.; Deng, Z. X., Gene cluster responsible for validamycin biosynthesis in Streptomyces hygroscopicus subsp jinggangensis 5008. (2005)Appl Environ Microb, 71 (9), 5066-76.

29. Wang, H.Y.*;Yu, Y.*, Chen, Z.L.; Xia, G.X. Functional characterization of Gossypium hirsutum profilin 1 gene (GhPFN1) in tobacco suspension cells. Characterization of in vivo functions of a cotton profilin gene. (2005)Planta222, 594-603. (*Co-First Author)

30.王岩；虞沂；赵群飞；孔毅；刘文，天然产物的生物合成和组合生物合成研究进展。国外医药（抗生素分册）2008，29（6），275-282

31.张华；赵群飞；虞沂；陆大年；刘文，特异性克隆编码多肽合成酶还原结构域基因的PCR方法。中国抗生素杂志2009，34（3），189-192

32. 吕美男；虞沂，2-脱氧链霉胺类抗生素的生物合成研究进展。中国科技论文在线, 2014

33. 张玉；虞沂，吡咯酰胺类化合物合成途径中醛基转移酶Pya26的功能研究。武汉大学学报（理学版），2016，62（1）：078-084

34. 黄胜；虞沂，二硫吡咯酮类抗生素的生物合成研究与代谢工程应用。微生物学报（微生物药物创新专刊），2016，3

专利

诺丝七肽的生物合成基因簇(专利号：ZL 2009 1 0053427.7授权公告日：2011年6月22日)

刘文；虞沂；段炼；雷春；丁莹；廖日晶

国际合作：

Dr. Hai Deng, Lecturer

University of Aberdeen, Department of Chemistry, Marine Biodiscovery Center